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10T / Kit A Rapid Test Kits For Vitamin D In Human Whole Blood , CE Certified

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Province/State: Zhejiang
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10T / Kit A Rapid Test Kits For Vitamin D In Human Whole Blood , CE Certified

Brand Name : AllTest
Model Number : cassette
Certification : CE
Place of Origin : CHINA
MOQ : 500
Supply Ability : 100 Million a year
Packaging Details : 10T/Kit
Format : Cassette
Specimen : whole blood
Kit Size : 10T/Kit
Cut-Off : 30ng/mL
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10T / Kit A Rapid Test Kits For Vitamin D In Human Whole Blood , CE Certified


The Vitamin D Rapid Test Cassette is a rapid chromatographic immunoassay for the semi-quantitative detection of total 25-hydroxy Vitamin D (25-OH Vitamin D) in human blood at a cut-off concentration of 30 ± 4ng/ml. This assay provides a preliminary diagnostic test result and can be used to screening for Vitamin D deficiency.


Vitamin D refers to a group of fat-soluble secosteroids responsible for increasing intestinal absorption of calcium, iron, magnesium, phosphate, and zinc. In humans, the most important compounds in this group are vitamin D3and vitamin D2.[1]Vitamin D3 is naturally produced in the human skin through the exposure to ultraviolet light and Vitamin D2 is mainly obtained from foods. Vitamin D is transported to the liver where it is metabolized to 25-hydroxy Vitamin D. In medicine, a 25-hydroxy Vitamin D blood test is used to determine Vitamin D concentration in the body. The blood concentration of 25-hydroxy Vitamin D (including D2 and D3) is considered the best indicator of Vitamin D status. Vitamin D deficiency is now recognized as a global epidemic. [2]Virtually every cell in our body has Receptors for Vitamin D, meaning that they all require “Sufficient” Level of Vitamin D for adequate functioning. The health risks associated with Vitamin D deficiency are far more severe than previously thought. Vitamin deficiency has been linked to various serious diseases: Osteoporosis, Osteomalacia, Multiple Sclerosis, Cardiovascular Diseases, Pregnancy Complications, Diabetes, Depression, Strokes, Autoimmune Diseases, Flu, Different Cancers, Infectious Diseases, Alzheimer, Obesity and Higher Mortality etc.[3] Therefore, now detecting (25-OH) Vitamin D level is considered as “Medically Necessary Screening Test”, and maintaining sufficient levels not just to improve bone health, but to improve overall health and well-being.[4]

How to use?

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing

For whole blood specimen:

1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible.

Place the cassette on a clean and level surface.

For Venipuncture Whole Blood specimen:

  • Hold the pipette vertically and transfer 20ml whole blood to the specimen well, then add 4 drops of buffer, and start the timer. See illustration below.

For Fingerstick Whole Blood specimen:

  • To use a Capillary droppers: Fill the capillary tube and transfer approximately 20ml of fingerstick whole blood specimen to the specimen area of test cassette, then add4 drops of buffer and start the timer. See illustration below.

2. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes.


(Please refer to the illustration)

≥30ng/ml: One line appears. Only control line appears(C).It depicts no Vitamin D deficiency.

<30ng/ml&>20ng/ml:Two lines appears(C/T), the intensity of T line is weaker than C line or the intensity of T line is similar to C line. The Vitamin D levels between 21-29 ng/ml signify Vitamin D insufficiency.

≤20ng/ml: Two lines appears(C/T), the intensity of T line is stronger than C line. This level depicts Vitamin D deficiency.

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.


A procedural control is included in the test. A colored line appearing in the control region (C) is the internal procedural control. It confirms sufficient specimen volume and correct procedural technique. Control standards are not supplied with this kit; however, it is recommended that positive and negative controls be tested as a good laboratory practice to confirm the test procedure and to verify proper test performance.

Product Tags:

rapid diagnostic test kits


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